NormalizePseudobulk
Usage
NormalizePseudobulk(
pseudobulklist,
normalization.method = "RLE",
design_matrix,
minimum.gene.count = 1
)Arguments
- pseudobulklist
object created with PseudobulkList only use this function if argument to PseudobulkList avg_or_sum was 'sum' computes normalization for pseudobulk libraries
- normalization.method
see edgeR function calcNormFactors, this is the argument to `method`
- design_matrix
the sample level metadata created by AggregateCellMetadata which will be used to make the designamatrix.
- minimum.gene.count
see edgeR function `filterbyExpr` thie is the argument to `min.count`